ROSA model using CRISPR

ROSA26 is a genomic mouse locus commonly used to knock-in cDNA constructs for ubiquitous or conditional gene expression in transgenic mice.  Working with the GEiC and using a previous  ROSA knock-in mouse model [CAG-floxed stop-cDNA-pA], we deleted the cDNA using the CRISPR system so that new cDNAs can be inserted directly between floxed stop and pA rather than having to insert the entire cassette for new ROSA KI’s.  This deletion-ROSA backbone method is more efficient than previous methods of generating ROSA knock-ins.