Services

Like traditional gene targeting in embryonic stem (ES) cells, the newest technology for inducing double-strand breaks (DSBs) is being utilized to make sophisticated molecular modifications to endogenous genes…

Cryopreservation of mouse sperm is offered to “store” lines of animals not currently needed, to reduce cage costs and cage space, or to provide secure stock in case of contamination. Two male mice are used…

The husbandry service includes all aspects of maintaining mice. Breeding, weaning, mouse monitoring, and tail biopsies are performed according to a standard operating procedure that has been used for 15 years with excellent results…

Animals that have known health risks must be kept in isolation and quarantine after importation. To reduce the time and increase the safety of the importation, we offer In Vitro Fertilization of imported…

Ovarian transplants are offered to maintain lines with normal ovaries but a phenotype that precludes breeding. The price includes transplant in up to four recipients from one donor female…

We perform genotyping by a universal polymerase chain reaction assay, including screens for induced mutations such as transgenes and ‘knockouts’ for colonies using our husbandry service. For breeding animals…

ROSA26 is a genomic mouse locus commonly used to knock-in cDNA constructs for ubiquitous or conditional gene expression in transgenic mice.  Working with the GEiC and using a previous…

Targeted mutations in one genetic background (for example, 129SV) frequently need to be transferred to another background more suitable for physiological analysis (for example, C57Bl6/J). This process takes…

Transgenic animals are produced by injecting a DNA construct directly into the pronucleus of a newly fertilized mouse egg. Plasmid or BAC constructs can be used. We routinely inject eggs from FVB/N…

Vasectomies are performed on any strain, and males are available for mating ten to twelve days after surgery. This price is per animal.